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ca 630 intercept tbs blocking buffer li cor  (LI-COR)


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    Structured Review

    LI-COR ca 630 intercept tbs blocking buffer li cor
    Ca 630 Intercept Tbs Blocking Buffer Li Cor, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 2135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+630+intercept+tbs+blocking+buffer+li+cor/Intercept+(TBS)+Blocking+Buffer/pm41832156-396-242-247
    Average 98 stars, based on 2135 article reviews
    ca 630 intercept tbs blocking buffer li cor - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.

    Hemagglutination Assay:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.

    Protease Inhibitor:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.

    Transcranial Magnetic Stimulation:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.

    Blocking Assay:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.

    Tissue:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.

    Control:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.

    Negative Control:

    Article Title: Control of lysosome function by the GTPase-activating protein TBC1D9B and its binding partner TMEM55B.
    Article Snippet: If not stated otherwise, analytical-grade chemicals were purchased from Sigma-Aldrich (MO, USA). .. The following primary antibodies were used: Reagent Source Identifier RRID Antibodies Actin Sigma Cat# A5441 RRID:AB_476744 AlexaFluor 488 anti-rat Thermo/ Molecular Probes Cat# A11006 RRID:AB_141373 AlexaFluor 594 anti-rabbit Thermo/ Molecular Probes Cat# A11037 RRID:AB_2534095 AlexaFluor 488 anti-mouse Thermo/ Molecular Probes Cat# A21202 RRID:AB_141607 AlexaFluor 647 anti-mouse Thermo/ Molecular Probes Cat# A21236 RRID:AB_2535805 AlexaFluor 568 anti-rabbit Thermo/ Molecular Probes Cat# A11036 RRID:AB_10563566 AlexaFluor 647 anti-rabbit Invitrogen Cat# A21244 RRID:AB_2535812 ARL8A/B Abcam Cat# ab281997 AR TI CL E IN P RE SS ARL8B Proteintech Cat# 13049-1-AP RRID:AB_2059000 Calnexin Proteintech Cat#10427-2-AP RRID:AB_2069033 CF647 anti-mouse Biotium Cat# 20281 RRID:AB_10853311 Cox IV (clone 3E11) Cell Signaling Cat# 4850 RRID:AB_2085424 EEA1 (clone C45B10 Cell Signaling Cat# 3288 RRID:AB_2096811 GFP (clone 7.1 + 13.1) Sigma Aldrich Cat#11814460001 RRID:AB_390913 HA (clone 3F10) Sigma Aldrich Cat# 11867423001 RRID:AB_390918 Halo Promega Cat# G9211 RRID:AB_2688011 IRDye 800CW anti-mouse LI-COR Cat# 926-32210 RRID:AB_621842 IRDye 800CW anti-rabbit LI-COR Cat# 926-32211 RRID:AB_621843 LAMP1 Cell Signaling Cat# 9091 RRID:AB_2687579 LAMP2 Abcam Cat# ab18528 RRID:AB_775981 LAMP2 (clone H4B4) DSHB Cat# sc-18822 RRID:AB_626858 LC3 MBL Cat# M152-3 RRID:AB_10949134 TBC1D9B Bethyl Laboratories Cat# A304-655A RRID:AB_2620850 TMEM55B Proteintech Cat# 23992-1-AP RRID:AB_2879391 RAB8 Cell Signaling Cat# 6975 RRID:AB_10827742 RAB11A Invitrogen Cat# 71-5300 RRID:AB_87868 Chemicals and Reagents Cell tracker green CMFDA Invitrogen Cat# C7025 cOmplete, Mini Protease Inhibitor Cocktail Roche Cat# 11836153001 Digitonin Invitrogen Cat# BN2006 FastDigest BpiI Thermo Cat# FD1014 AR TI CL E IN P RE SS GFP-Trap Magnetic Agarose Proteintech Cat# gtma Goat serum Gibco Cat# 16210072 Igepal Sigma-Aldrich Cat# CA-630 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 INTERFERin VWR Cat# 101000028 jetPrime VWR Cat# 101000001 Matrigel Corning Cat# 356231 NucleoSpin Tissue Macherey-Nagel Cat# 740952.250 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 Puromycin Gibco Cat# A1113803 Saponin Sigma Cat# 47036 Shandon Immu-Mount Epredia Cat# 9990402 Oligonucleotides (siRNA) siARL8B Dharmacon/Horizon Cat# L-031872-02 Sigma scr control Sigma Mission siRNA universal negative control #1 Cat# SIC001 siTBC1D9B Dharmacon/Horizon Cat# L-020294-01 .. U2OS and HeLa cells were sourced from ATCC.



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    LI-COR ca 630 intercept tbs blocking buffer li cor
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    https://www.bioz.com/product/ca+630+intercept+tbs+blocking+buffer+li+cor/Intercept+(TBS)+Blocking+Buffer/pm41832156-396-242-247
    Average 98 stars, based on 1 article reviews
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